PMID:15710381

Citation	Croitoru, VV, Bucheli-Witschel, M and Isaksson, LA (2005) In vivo involvement of mutated initiation factor IF1 in gene expression control at the translational level. FEBS Lett. 579:995-1000
Abstract	The influence in vivo of mutated forms of translation initiation factor (IF1) on the expression of the lacZ or 3A' reporter genes, with different initiation and/or +2 codons, has been investigated. Reporter gene expression in these infA(IF1) mutants is similar to the wild-type strain. The results do not support the longstanding hypothesis that IF1 could perform discriminatory functions while blocking the aminoacyl-tRNA acceptor site (A-site) of the ribosome. One cold-sensitive IF1 mutant shows a general overexpression, in particular at low temperatures, of both reporter genes at the protein but not mRNA level.
Links	PubMed Online version:10.1016/j.febslet.2004.12.072
Keywords	Codon/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation/genetics; Genes, Reporter/genetics; Genetic Variation/genetics; Lac Operon/genetics; Mutation/genetics; Prokaryotic Initiation Factor-1/genetics; Prokaryotic Initiation Factor-1/metabolism; Protein Biosynthesis/genetics; RNA, Messenger/genetics; RNA, Messenger/metabolism; Temperature
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.