PMID:15705577
| Citation |
Liu, M, Durfee, T, Cabrera, JE, Zhao, K, Jin, DJ and Blattner, FR (2005) Global transcriptional programs reveal a carbon source foraging strategy by Escherichia coli. J. Biol. Chem. 280:15921-7 |
|---|---|
| Abstract |
By exploring global gene expression of Escherichia coli growing on six different carbon sources, we discovered a striking genome transcription pattern: as carbon substrate quality declines, cells systematically increase the number of genes expressed. Gene induction occurs in a hierarchical manner and includes many factors for uptake and metabolism of better but currently unavailable carbon sources. Concomitantly, cells also increase their motility. Thus, as the growth potential of the environment decreases, cells appear to devote progressively more energy on the mere possibility of improving conditions. This adaptation is not what would be predicated by classic regulatory models alone. We also observe an inverse correlation between gene activation and rRNA synthesis suggesting that reapportioning RNA polymerase (RNAP) contributes to the expanded genome activation. Significant differences in RNAP distribution in vivo, monitored using an RNAP-green fluorescent protein fusion, from energy-rich and energy-poor carbon source cultures support this hypothesis. Together, these findings represent the integration of both substrate-specific and global regulatory systems, and may be a bacterial approximation to metazoan risk-prone foraging behavior. |
| Links |
PubMed Online version:10.1074/jbc.M414050200 |
| Keywords |
Acetic Acid/metabolism; Alanine/metabolism; Carbon/metabolism; Computer Simulation; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Profiling; Glucose/metabolism; Glycerol/metabolism; Models, Biological; Proline/metabolism; Promoter Regions, Genetic; RNA, Ribosomal/metabolism; Succinic Acid/metabolism; Transcription, Genetic; Up-Regulation |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<protect><annotationlinks/></protect>
<protect>
| Accessions | SMD Category | SMD Subcategory | SMD status |
|---|---|---|---|
| edit table |
</protect>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
