PMID:1569010
| Citation |
Lin, R, Ernsting, B, Hirshfield, IN, Matthews, RG, Neidhardt, FC, Clark, RL and Newman, EB (1992) The lrp gene product regulates expression of lysU in Escherichia coli K-12. J. Bacteriol. 174:2779-84 |
|---|---|
| Abstract |
In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that LysU is present at higher levels in an lrp strain than in its isogenic lrp+ parent. The purified lrp gene product was shown to bind to sites upstream of the lysU gene and to protect several sites against DNase I digestion. A region extending over 100 nucleotides, between 60 and 160 nucleotides upstream from the start of the lysU coding sequence, showed altered sensitivity to DNase I digestion in the presence of the Lrp protein. The extent of protected DNA suggests a complex interaction of Lrp protein and upstream lysU DNA. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; DNA, Bacterial/genetics; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial/genetics; Leucine-Responsive Regulatory Protein; Lysine-tRNA Ligase/genetics; Molecular Sequence Data; Mutagenesis; Protein Biosynthesis; Regulatory Sequences, Nucleic Acid/genetics; Transcription Factors |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.