PMID:15685598

Citation	Engel, S, Vyazmensky, M, Berkovich, D, Barak, Z, Merchuk, J and Chipman, DM (2005) Column flow reactor using acetohydroxyacid synthase I from Escherichia coli as catalyst in continuous synthesis of R-phenylacetyl carbinol. Biotechnol. Bioeng. 89:733-40
Abstract	We tested the possibility of utilizing acetohydroxyacid synthase I (AHAS I) from Escherichia coli in a continuous flow reactor for production of R-phenylacetyl carbinol (R-PAC). We constructed a fusion of the large, catalytic subunit of AHAS I with a cellulose binding domain (CBD). This allowed purification of the enzyme and its immobilization on cellulose in a single step. After immobilization, AHAS I is fully active and can be used as a catalyst in an R-PAC production unit, operating either in batch or continuous mode. We propose a simplified mechanistic model that can predict the product output of the AHAS I-catalyzed reaction. This model should be useful for optimization and scaling up of a R-PAC production unit, as demonstrated by a column flow reactor.
Links	PubMed Online version:10.1002/bit.20392
Keywords	Acetolactate Synthase/chemistry; Acetolactate Synthase/isolation & purification; Acetolactate Synthase/metabolism; Acetone/analogs & derivatives; Acetone/metabolism; Bioreactors/microbiology; Catalysis; Cellulose/chemistry; Enzymes, Immobilized/biosynthesis; Escherichia coli/enzymology; Protein Structure, Tertiary
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.