PMID:15681387

Citation	Pease, PJ, Levy, O, Cost, GJ, Gore, J, Ptacin, JL, Sherratt, D, Bustamante, C and Cozzarelli, NR (2005) Sequence-directed DNA translocation by purified FtsK. Science 307:586-90
Abstract	DNA translocases are molecular motors that move rapidly along DNA using adenosine triphosphate as the source of energy. We directly observed the movement of purified FtsK, an Escherichia coli translocase, on single DNA molecules. The protein moves at 5 kilobases per second and against forces up to 60 piconewtons, and locally reverses direction without dissociation. On three natural substrates, independent of its initial binding position, FtsK efficiently translocates over long distances to the terminal region of the E. coli chromosome, as it does in vivo. Our results imply that FtsK is a bidirectional motor that changes direction in response to short, asymmetric directing DNA sequences.
Links	PubMed Online version:10.1126/science.1104885
Keywords	Algorithms; Bacteriophage lambda; Base Sequence; Chromosomes, Bacterial; DNA, Bacterial/chemistry; DNA, Bacterial/metabolism; DNA, Superhelical/chemistry; DNA, Superhelical/metabolism; DNA, Viral/chemistry; DNA, Viral/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins/isolation & purification; Escherichia coli Proteins/metabolism; Kinetics; Membrane Proteins/isolation & purification; Membrane Proteins/metabolism; Models, Biological; Molecular Motor Proteins/isolation & purification; Molecular Motor Proteins/metabolism; Nucleic Acid Conformation
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