PMID:15665866
| Citation |
Edwards, MD, Li, Y, Kim, S, Miller, S, Bartlett, W, Black, S, Dennison, S, Iscla, I, Blount, P, Bowie, JU and Booth, IR (2005) Pivotal role of the glycine-rich TM3 helix in gating the MscS mechanosensitive channel. Nat. Struct. Mol. Biol. 12:113-9 |
|---|---|
| Abstract |
The crystal structure of an open form of the Escherichia coli MscS mechanosensitive channel was recently solved. However, the conformation of the closed state and the gating transition remain uncharacterized. The pore-lining transmembrane helix contains a conserved glycine- and alanine-rich motif that forms a helix-helix interface. We show that introducing 'knobs' on the smooth glycine face by replacing glycine with alanine, and substituting conserved alanines with larger residues, increases the pressure required for gating. Creation of a glycine-glycine interface lowers activation pressure. The importance of residues Gly104, Ala106 and Gly108, which flank the hydrophobic seal, is demonstrated. A new structural model is proposed for the closed-to-open transition that involves rotation and tilt of the pore-lining helices. Introduction of glycine at Ala106 validated this model by acting as a powerful suppressor of defects seen with mutations at Gly104 and Gly108. |
| Links |
PubMed Online version:10.1038/nsmb895 |
| Keywords |
Alanine/genetics; Alanine/metabolism; Electrophysiology; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Glycine/genetics; Glycine/metabolism; Ion Channels/chemistry; Ion Channels/genetics; Ion Channels/metabolism; Models, Molecular; Mutation/genetics; Phenotype; Protein Structure, Tertiary |
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