PMID:15647255
| Citation |
Bruck, I, Georgescu, RE and O'Donnell, M (2005) Conserved interactions in the Staphylococcus aureus DNA PolC chromosome replication machine. J. Biol. Chem. 280:18152-62 |
|---|---|
| Abstract |
The PolC holoenzyme replicase of the Gram-positive Staphylococcus aureus pathogen has been reconstituted from pure subunits. We compared individual S. aureus replicase subunits with subunits from the Gram-negative Escherichia coli polymerase III holoenzyme for activity and interchangeability. The central organizing subunit, tau, is smaller than its Gram-negative homolog, yet retains the ability to bind single-stranded DNA and contains DNA-stimulated ATPase activity comparable with E. coli tau. S. aureus tau also stimulates PolC, although they do not form as stabile a complex as E. coli polymerase III.tau. We demonstrate that the extreme C-terminal residues of PolC bind to and function with beta clamps from different bacteria. Hence, this polymerase-clamp interaction is highly conserved. Additionally, the S. aureus delta wrench of the clamp loader binds to E. coli beta. The S. aureus clamp loader is even capable of loading E. coli and Streptococcus pyogenes beta clamps onto DNA. Interestingly, S. aureus PolC lacks functionality with heterologous beta clamps when they are loaded onto DNA by the S. aureus clamp loader, suggesting that the S. aureus clamp loader may have difficulty ejecting from heterologous clamps. Nevertheless, these overall findings underscore the conservation in structure and function of Gram-positive and Gram-negative replicases despite >1 billion years of evolutionary distance between them. |
| Links |
PubMed Online version:10.1074/jbc.M413595200 |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Chromosomes/genetics; Conserved Sequence/genetics; DNA Replication/genetics; DNA Replication Timing/genetics; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Directed DNA Polymerase/genetics; DNA-Directed DNA Polymerase/metabolism; Staphylococcus aureus/enzymology; Staphylococcus aureus/genetics |
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