PMID:15618641
| Citation |
Shibuya, A, Tsukagoshi, N, Ohtsu, I, Dokyu, N and Aono, R (2004) Convenient and sensitive evaluation of a superoxide anion-generating reagent methyl viologen by Escherichia coli harboring a soxS'::gfp reporter plasmid. Biosci. Biotechnol. Biochem. 68:2637-9 |
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| Abstract |
We constructed a reporter system to detect a superoxide-generating methyl viologen using SoxRS of Escherichia coli and GFP of Aequorea victoria. E. coli carrying this plasmid exhibited strong fluorescence when grown in the presence of a superoxide-generating reagent methyl viologen. The fluorescence intensity observed in the stationary phase culture of the transformant increased in response to the methyl viologen concentration in a range of 0.01 microM to 10 microM. |
| Links | |
| Keywords |
Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Fluorescence; Genes, Reporter; Green Fluorescent Proteins/genetics; Indicators and Reagents; Methods; Paraquat/analysis; Superoxides/chemistry; Trans-Activators/genetics; Transduction, Genetic |
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