PMID:15587
| Citation |
Applebaum, DM, Dunlap, JC and Morris, DR (1977) Comparison of the biosynthetic and biodegradative ornithine decarboxylases of Escherichia coli. Biochemistry 16:1580-4 |
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| Abstract |
Biosynthetic ornithine decarboxylase was purified 4300-fold from Escherichia coli to a purity of approximately 85% as judged by polyacrylamide gel electrophoresis. The enzyme showed hyperbolic kinetics with a Km of 5.6 mM for ornithine and 1.0 micronM for pyridoxal phosphate and it was competitively inhibited by putrescine and spermidine. The biosynthetic decarboxylase was compared with the biodegradative ornithine decarboxylase [Applebaum, D., et al. (1975), Biochemistry 14, 3675]. Both enzymes were dimers of 80 000-82 000 molecular weight and exhibited similar kinetic properties. However, they differed significantly in other respects. The pH optimum of the biosynthetic enzyme was 8.1, compared with 6.9 for the biodegradative. Both enzymes were activated by nucleotides, but with different specificity. Antibody to the purified biodegradative ornithine decarboxylase did not cross-react with the biosynthetic enzyme. The evolutionary relationship of these two decarboxylases to the other amino acid decarboxylases of E. coli is discussed. |
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| Keywords |
Carboxy-Lyases/metabolism; Chromatography; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Hydrogen-Ion Concentration; Hydroxyapatites; Immunodiffusion; Isoenzymes/isolation & purification; Isoenzymes/metabolism; Kinetics; Molecular Weight; Ornithine Decarboxylase/isolation & purification; Ornithine Decarboxylase/metabolism; Putrescine/pharmacology; Ribonucleotides/pharmacology; Spermidine/pharmacology |
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