PMID:15574802

Citation	Nita-Lazar, M, Wacker, M, Schegg, B, Amber, S and Aebi, M (2005) The N-X-S/T consensus sequence is required but not sufficient for bacterial N-linked protein glycosylation. Glycobiology 15:361-7
Abstract	In the Gram-negative bacterium Campylobacter jejuni there is a pgl (protein glycosylation) locus-dependent general N-glycosylation system of proteins. One of the proteins encoded by pgl locus, PglB, a homolog of the eukaryotic oligosaccharyltransferase component Stt3p, is proposed to function as an oligosaccharyltransferase in this prokaryotic system. The sequence requirements of the acceptor polypeptide for N-glycosylation were analyzed by reverse genetics using the reconstituted glycosylation of the model protein AcrA in Escherichia coli. As in eukaryotes, the N-X-S/T sequon is an essential but not a sufficient determinant for N-linked protein glycosylation. This conclusion was supported by the analysis of a novel C. jejuni glycoprotein, HisJ. Export of the polypeptide to the periplasm was required for glycosylation. Our data support the hypothesis that eukaryotic and bacterial N-linked protein glycosylation are homologous processes.
Links	PubMed Online version:10.1093/glycob/cwi019
Keywords	Amino Acid Motifs/genetics; Bacterial Proteins/genetics; Campylobacter jejuni/genetics; Glycosylation; Hexosyltransferases/genetics; Membrane Proteins/genetics; Protein Processing, Post-Translational/genetics; Protein Transport/genetics
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