PMID:15568015
| Citation |
Conroy, MJ, Jamieson, SJ, Blakey, D, Kaufmann, T, Engel, A, Fotiadis, D, Merrick, M and Bullough, PA (2004) Electron and atomic force microscopy of the trimeric ammonium transporter AmtB. EMBO Rep. 5:1153-8 |
|---|---|
| Abstract |
Escherichia coli AmtB is an archetypal member of the ammonium transporter (Amt) family, a family of proteins that are conserved in all domains of life. Reconstitution of AmtB in the presence of lipids produced large, ordered two-dimensional crystals. From these, a 12 A resolution projection map was determined by cryoelectron microscopy, and high-resolution topographs were acquired using atomic force microscopy. Both techniques showed the trimeric structure of AmtB in which each monomer seems to have a pseudo-two-fold symmetry. This arrangement is likely to represent the in vivo structure. This work provides the first views of the structure of any member of the Amt family. |
| Links |
PubMed PMC1299191 Online version:10.1038/sj.embor.7400296 |
| Keywords |
Cation Transport Proteins/chemistry; Cryoelectron Microscopy; Crystallization; Escherichia coli; Escherichia coli Proteins/chemistry; Microscopy, Atomic Force |
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