PMID:15556557

Citation	Pavel, I, Belcher, A and Browning, KS (2004) A method for coupled transcription and aminoacylation of cysteinyl-tRNA. Anal. Biochem. 335:192-5
Abstract	A novel method for coupled transcription and aminoacylation of transfer RNA was developed where Escherichia coli cysteine-specific tRNA (tRNA(cys)) was transcribed and aminoacylated in a single reaction. The cys-tRNA(cys) that was synthesized and aminoacylated using this method was functional in in vitro translation. The cys-tRNA(cys) was further modified with biotin (N-iodoacetyl-N-biotinhexylenediamine) to facilitate detection. The biotin-modified cys-tRNAs(cys) was also functional in in vitro translation, allowing the synthesis and detection of biotin-labeled protein.
Links	PubMed Online version:10.1016/j.ab.2004.08.029
Keywords	Aminoacylation; Biotin; Escherichia coli/genetics; Protein Biosynthesis; RNA, Bacterial/genetics; RNA, Transfer, Amino Acyl/chemistry; RNA, Transfer, Amino Acyl/genetics; Transcription, Genetic
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