PMID:15546870
| Citation |
Justino, MC, Vicente, JB, Teixeira, M and Saraiva, LM (2005) New genes implicated in the protection of anaerobically grown Escherichia coli against nitric oxide. J. Biol. Chem. 280:2636-43 |
|---|---|
| Abstract |
Nitric oxide produced by activated macrophages plays a key role as one of the immune system's weapons against pathogens. Because the lifetime of nitric oxide is short in aerobic conditions, whereas in anaerobic conditions the cytotoxic effects of nitric oxide are greatly increased as in the infection/inflammation processes, it is important to establish which systems are able to detoxify nitric oxide under anaerobic conditions. In the present work a new set of Escherichia coli K-12 genes conferring anaerobic resistance to nitric oxide is presented, namely the gene product of YtfE and a potential transcriptional regulator of the helix-turn-helix LysR-type (YidZ). The crucial role of flavohemoglobin for anaerobic nitric oxide protection is also demonstrated. Furthermore, nitric oxide is shown to cause a significant alteration of the global E. coli gene transcription profile that includes the increase of the transcript level of genes encoding for detoxification enzymes, iron-sulfur cluster assembly systems, DNA-repairing enzymes, and stress response regulators. |
| Links |
PubMed Online version:10.1074/jbc.M411070200 |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; DNA/chemistry; DNA Repair; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Hemeproteins/chemistry; Macrophages/metabolism; Molecular Sequence Data; Mutation; Nitric Oxide/chemistry; Nitric Oxide/metabolism; Oligonucleotide Array Sequence Analysis; RNA/chemistry; RNA/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Time Factors; Transcription Factors/chemistry; Transcription Factors/genetics; Transcription, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.