PMID:15542561
| Citation |
Wiggins, P and Phillips, R (2005) Membrane-protein interactions in mechanosensitive channels. Biophys. J. 88:880-902 |
|---|---|
| Abstract |
In this article, we examine the mechanical role of the lipid bilayer in ion channel conformation and function with specific reference to the case of the mechanosensitive channel of large conductance (MscL). In a recent article we argued that mechanotransduction very naturally arises from lipid-protein interactions by invoking a simple analytic model of the MscL channel and the surrounding lipid bilayer. In this article, we focus on improving and expanding this analytic framework for studying lipid-protein interactions with special attention to MscL. Our goal is to generate simple scaling relations which can be used to provide qualitative understanding of the role of membrane mechanics in protein function and to quantitatively interpret experimental results. For the MscL channel, we find that the free energies induced by lipid-protein interaction are of the same order as the measured free energy differences between conductance states. We therefore conclude that the mechanics of the bilayer plays an essential role in determining the conformation and function of the channel. Finally, we compare the predictions of our model to experimental results from the recent investigations of the MscL channel by a variety of investigators and suggest a suite of new experiments. |
| Links |
PubMed PMC1305162 Online version:10.1529/biophysj.104.047431 |
| Keywords |
Binding Sites; Cell Membrane/chemistry; Cell Membrane/physiology; Computer Simulation; Energy Transfer/physiology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/physiology; Ion Channel Gating/physiology; Ion Channels/chemistry; Ion Channels/physiology; Lipid Bilayers/chemistry; Mechanotransduction, Cellular/physiology; Membrane Fluidity/physiology; Models, Biological; Models, Chemical; Protein Binding; Stress, Mechanical |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.