PMID:15531892
| Citation |
Mikulecky, PJ, Kaw, MK, Brescia, CC, Takach, JC, Sledjeski, DD and Feig, AL (2004) Escherichia coli Hfq has distinct interaction surfaces for DsrA, rpoS and poly(A) RNAs. Nat. Struct. Mol. Biol. 11:1206-14 |
|---|---|
| Abstract |
The bacterial Sm-like protein Hfq facilitates RNA-RNA interactions involved in post-transcriptional regulation of the stress response. Specifically, Hfq helps pair noncoding RNAs (ncRNAs) with complementary regions of target mRNAs. To probe the mechanism of this pairing, we generated a series of Hfq mutants and measured their affinity for RNAs like those with which Hfq must associate in vivo. We tested the mutants' DsrA-dependent activation of rpoS, and their ability to stabilize DsrA ncRNA against degradation in vivo. Our results suggest that Hfq has two independent RNA-binding surfaces. In addition to a well-known site around the core of the Hfq hexamer, we observe interactions with the distal face of Hfq, a new locus with which mRNAs and poly(A) sequences associate. Our model explains how Hfq can simultaneously bind a ncRNA and its mRNA target to facilitate the strand displacement reaction required for Hfq-dependent translational regulation. |
| Links |
PubMed PMC3071270 Online version:10.1038/nsmb858 |
| Keywords |
Amino Acid Sequence; Bacterial Outer Membrane Proteins/metabolism; Bacterial Proteins/metabolism; Binding, Competitive; Calorimetry; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Host Factor 1 Protein/chemistry; Host Factor 1 Protein/genetics; Host Factor 1 Protein/metabolism; Models, Molecular; Molecular Sequence Data; Mutation/genetics; Poly A/chemistry; Poly A/metabolism; Protein Denaturation; Protein Structure, Tertiary; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA-Binding Proteins/chemistry; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Sequence Alignment; Sigma Factor/metabolism; Titrimetry |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.