PMID:15498773
| Citation |
Rancour, DM, Park, S, Knight, SD and Bednarek, SY (2004) Plant UBX domain-containing protein 1, PUX1, regulates the oligomeric structure and activity of arabidopsis CDC48. J. Biol. Chem. 279:54264-74 |
|---|---|
| Abstract |
p97/CDC48 is a highly abundant hexameric AAA-ATPase that functions as a molecular chaperone in numerous diverse cellular activities. We have identified an Arabidopsis UBX domain-containing protein, PUX1, which functions to regulate the oligomeric structure of the Arabidopsis homolog of p97/CDC48, AtCDC48, as well as mammalian p97. PUX1 is a soluble protein that co-fractionates with non-hexameric AtCDC48 and physically interacts with AtCDC48 in vivo. Binding of PUX1 to AtCDC48 is mediated through the UBX-containing C-terminal domain. However, disassembly of the chaperone is dependent upon the N-terminal domain of PUX1. These findings provide evidence that the assembly and disassembly of the hexameric p97/CDC48 complex is a dynamic process. This new unexpected level of regulation for p97/CDC48 was demonstrated to be critical in vivo as pux1 loss-of-function mutants display accelerated growth relative to wild-type plants. These results suggest a role for AtCDC48 and PUX1 in regulating plant growth. |
| Links |
PubMed Online version:10.1074/jbc.M405498200 |
| Keywords |
Adenosine Triphosphatases; Amino Acid Sequence; Animals; Arabidopsis/chemistry; Arabidopsis/embryology; Arabidopsis/growth & development; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/physiology; Binding Sites; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/physiology; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Chromatography, Affinity; Escherichia coli/genetics; Gene Expression; Immunoblotting; Kinetics; Mice; Molecular Sequence Data; Molecular Structure; Mutagenesis; Peptide Fragments/chemistry; Peptide Fragments/genetics; Peptide Fragments/metabolism; Plant Roots/growth & development; Polymerase Chain Reaction; Recombinant Fusion Proteins; Seeds/growth & development; Sequence Alignment; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure-Activity Relationship |
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