PMID:15496390
| Citation |
Tavío, MM, Vila, J, Perilli, M, Casañas, LT, Maciá, L, Amicosante, G and Jiménez de Anta, MT (2004) Enhanced active efflux, repression of porin synthesis and development of Mar phenotype by diazepam in two enterobacteria strains. J. Med. Microbiol. 53:1119-22 |
|---|---|
| Abstract |
The aim of this work was to determine whether diazepam could induce the multiple antibiotic resistance (Mar) phenotype in Klebsiella pneumoniae and Escherichia coli strains. The Mar phenotype is characterized by decreased susceptibility to multiple antibiotics due to the loss of porins and/or increased expression of active efflux systems. The effect of subinhibitory concentrations of diazepam on the susceptibility of different antimicrobial agents, outer-membrane protein expression and norfloxacin intracellular accumulation was studied. The results revealed that diazepam concentrations equal or twice adult dosage induced the same Mar phenotype as two well known E. coli marRAB inducers, sodium salicylate and sodium benzoate. Susceptibility to norfloxacin in a K. pneumoniae clinical isolate and E. coli strain Ag100 decreased due to enhanced active efflux and loss of porin expression. A decreased susceptibility to chloramphenicol, tetracycline, nalidixic acid and beta-lactam antibiotics was also observed. In conclusion, like sodium salicylate or sodium benzoate, diazepam may induce the Mar phenotype. |
| Links | |
| Keywords |
Anti-Bacterial Agents/pharmacology; Bacterial Outer Membrane Proteins/biosynthesis; Biological Transport, Active/drug effects; Cytoplasm/chemistry; Diazepam/pharmacology; Drug Resistance, Multiple, Bacterial/drug effects; Enzyme Activators/pharmacology; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial/drug effects; Klebsiella pneumoniae/drug effects; Klebsiella pneumoniae/genetics; Klebsiella pneumoniae/metabolism; Microbial Sensitivity Tests; Norfloxacin/metabolism; Norfloxacin/pharmacology; Porins/biosynthesis; Sodium Benzoate/pharmacology; Sodium Salicylate/pharmacology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.