PMID:15476399

Citation	Sun, W, Li, G and Nicholson, AW (2004) Mutational analysis of the nuclease domain of Escherichia coli ribonuclease III. Identification of conserved acidic residues that are important for catalytic function in vitro. Biochemistry 43:13054-62
Abstract	The ribonuclease III superfamily represents a structurally distinct group of double-strand-specific endonucleases with essential roles in RNA maturation, RNA decay, and gene silencing. Bacterial RNase III orthologs exhibit the simplest structures, with an N-terminal nuclease domain and a C-terminal double-stranded RNA-binding domain (dsRBD), and are active as homodimers. The nuclease domain contains conserved acidic amino acids, which in Escherichia coli RNase III are E38, E41, D45, E65, E100, D114, and E117. On the basis of a previously reported crystal structure of the nuclease domain of Aquifex aeolicus RNase III, the E41, D114, and E117 side chains of E. coli RNase III are expected to be coordinated to a divalent metal ion (Mg(2+) or Mn(2+)). It is shown here that the RNase III[E41A] and RNase III[D114A] mutants exhibit catalytic activities in vitro in 10 mM Mg(2+) buffer that are comparable to that of the wild-type enzyme. However, at 1 mM Mg(2+), the activities are significantly lower, which suggests a weakened affinity for metal. While RNase III[E41A] and RNase III[D114A] have K(Mg) values that are approximately 2.8-fold larger than the K(Mg) of RNase III (0.46 mM), the RNase III[E41A/D114A] double mutant has a K(Mg) of 39 mM, suggesting a redundant function for the two side chains. RNase III[E38A], RNase III[E65A], and RNase III[E100A] also require higher Mg(2+) concentrations for optimal activity, with RNase III[E100A] exhibiting the largest K(Mg). RNase III[D45A], RNase III[D45E], and RNase III[D45N] exhibit negligible activities, regardless of the Mg(2+) concentration, indicating a stringent functional requirement for an aspartate side chain. RNase III[D45E] activity is partially rescued by Mn(2+). The potential functions of the conserved acidic residues are discussed in the context of the crystallographic data and proposed catalytic mechanisms.
Links	PubMed Online version:10.1021/bi049258i
Keywords	Alanine/genetics; Amino Acid Sequence; Aspartic Acid/genetics; Base Sequence; Catalysis; Catalytic Domain/genetics; Conserved Sequence; DNA Mutational Analysis; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Glutamic Acid/genetics; Hydrogen-Ion Concentration; Hydrolysis; Manganese/chemistry; Molecular Sequence Data; Protein Subunits/chemistry; Protein Subunits/genetics; Ribonuclease III/chemistry; Ribonuclease III/genetics; Sequence Alignment; Sequence Homology, Amino Acid
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