PMID:1547498

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Citation

Sullivan, SL and Gottesman, ME (1992) Requirement for E. coli NusG protein in factor-dependent transcription termination. Cell 68:989-94

Abstract

The 21 kd NusG protein is essential for E. coli viability. Cells depleted for NusG were defective for factor-dependent transcription termination. Rho-induced polarity in the gal operon and the Rho-dependent lambda tR1 and lambda tL1 terminators were suppressed in NusG-deficient cells. NusG depletion inactivated the phage HK022 Nun termination factor. In contrast, the factor-independent lambda tl terminator was fully active in NusG-depleted cells and could be suppressed by phage lambda N function.

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Keywords

Bacterial Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Peptide Elongation Factors/metabolism; Transcription Factors/metabolism; Transcription, Genetic

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