PMID:15474015

Citation	Stevens, JM, Gordon, EH and Ferguson, SJ (2004) Overproduction of CcmABCDEFGH restores cytochrome c maturation in a DsbD deletion strain of E. coli: another route for reductant? FEBS Lett. 576:81-5
Abstract	The multidomain transmembrane protein DsbD is essential for cytochrome c maturation (Ccm) in Escherichia coli and transports reductant to the otherwise oxidising environment of the bacterial periplasm. The Ccm proteins ABCDEFGH are also essential and we show that the overproduction of these proteins can unexpectedly complement for the absence of DsbD in a deletion strain by partially restoring the production of an exogenous c-type cytochrome under aerobic and anaerobic conditions. This suggests that one or more of the Ccm proteins can provide reductant to the periplasm. The Ccm proteins do not, however, restore the normal disulfide mis-isomerisation phenotype of the deletion strain, as shown by assay of the multidisulfide-bonded enzyme urokinase.
Links	PubMed Online version:10.1016/j.febslet.2004.08.067
Keywords	Aerobiosis; Anaerobiosis; Cytochromes c/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/biosynthesis; Gene Deletion; Genes, Bacterial; Genetic Complementation Test; Protein Disulfide-Isomerases/genetics; Urokinase-Type Plasminogen Activator/analysis; Urokinase-Type Plasminogen Activator/metabolism
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