PMID:15469740

Citation	Lee, HC, Lee, SD, Sohng, JK and Liou, K (2004) One-pot enzymatic synthesis of UDP-D-glucose from UMP and glucose-1-phosphate using an ATP regeneration system. J. Biochem. Mol. Biol. 37:503-6
Abstract	Glucose-1-phosphate uridylyltransferase from E. coli K12 was used to convert uridine-5'-triphosphate and glucose-1-phosphate to UDP-D-glucose. The conversion was efficient and completed within 5 minutes under the employed conditions. In addition, thymidine-5'-monophosphate kinase and acetate kinase were proven to be non-specific, converting udridine-5'-monophosphate to uridine-5'-triphosphate with 55% conversion after 6 h, which was much slower than the production of TTP under the same conditions (complete conversion within one hour). Since these two reactions could proceed under the same conditions, a one-pot synthesis of UDP-D-glucose with ATP regeneration was designed from easily available starting materials, and conversion up to 40% by HPLC peak integration was achieved given a reaction time of 4 h.
Links	PubMed
Keywords	Adenosine Triphosphate/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Glucosephosphates/metabolism; Molecular Structure; UTP-Glucose-1-Phosphate Uridylyltransferase/genetics; UTP-Glucose-1-Phosphate Uridylyltransferase/metabolism; Uridine Diphosphate Glucose/chemistry; Uridine Diphosphate Glucose/metabolism; Uridine Monophosphate/metabolism
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