PMID:15466585

Citation	Suzuki, H, Miwa, C, Ishihara, S and Kumagai, H (2004) A single amino acid substitution converts gamma-glutamyltranspeptidase to a class IV cephalosporin acylase (glutaryl-7-aminocephalosporanic acid acylase). Appl. Environ. Microbiol. 70:6324-8
Abstract	The aspartyl residue at position 433 of gamma-glutamyltranspeptidase of Escherichia coli K-12 was replaced by an asparaginyl residue. This substitution enabled gamma-glutamyltranspeptidase to deacylate glutaryl-7-aminocephalosporanic acid, producing 7-aminocephalosporanic acid, which is a starting material for the synthesis of semisynthetic cephalosporins.
Links	PubMed PMC522061 Online version:10.1128/AEM.70.10.6324-6328.2004
Keywords	Amino Acid Substitution; Base Sequence; DNA, Bacterial/genetics; Escherichia coli K12/enzymology; Escherichia coli K12/genetics; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Mutagenesis, Site-Directed; Penicillin Amidase/chemistry; Penicillin Amidase/genetics; Penicillin Amidase/metabolism; Substrate Specificity; gamma-Glutamyltransferase/chemistry; gamma-Glutamyltransferase/genetics; gamma-Glutamyltransferase/metabolism
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