PMID:15450297

Citation	Gao, H, Valle, M, Ehrenberg, M and Frank, J (2004) Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM dataset. J. Struct. Biol. 147:283-90
Abstract	A method of supervised classification using two available structure templates was applied to investigate the possible heterogeneity existing in a large cryo-EM dataset of an Escherichia coli 70S ribosome-EF-G complex. Two subpopulations showing the ribosome in distinct conformational states, related by a ratchet-like rotation of the 30S subunit with respect to the 50S subunit, were extracted from the original dataset. The possible presence of additional intermediate states is discussed.
Links	PubMed Online version:10.1016/j.jsb.2004.02.008
Keywords	Cryoelectron Microscopy/methods; Escherichia coli/metabolism; Escherichia coli/ultrastructure; Fourier Analysis; Models, Molecular; Peptide Elongation Factor G/chemistry; Peptide Elongation Factor G/metabolism; Protein Conformation; RNA, Bacterial/chemistry; RNA, Bacterial/metabolism; RNA, Transfer/chemistry; RNA, Transfer/metabolism; Ribosomes/metabolism; Ribosomes/ultrastructure
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