PMID:1543701
| Citation |
Unemoto, T, Shimada, H and Hayashi, M (1992) Chemical structures critical for the induction of FMN-dependent NADH-quinone reductase in Escherichia coli. Biochim. Biophys. Acta 1099:170-4 |
|---|---|
| Abstract |
An FMN-dependent NADH-quinone reductase is induced in Escherichia coli by growing the cells in the presence of menadione (2-methyl-1,4-naphthoquinone). Since the properties of induced enzyme are very similar to those of NAD(P)H: (quinone-acceptor) oxidoreductase (EC 1.6.99.2), known as DT-diaphorase, from animal cells, structural requirements of quinone derivatives as an inducer of NADH-quinone reductase in E. coli were examined. Among quinone derivatives examined, it was found that 2-alkyl-1,4-quinone structure with C-3 unsubstituted or substituted with Br is critical as a common inductive signal. Michael reaction acceptors which have been reported to be strong inducers of DT-diaphorase in mouse hepatoma cells were not always effective inducers in E. coli. However, several compounds, such as 2-methylene-4-butyrolactone, methylacrylate and methyl vinyl ketone, showed a slight inductive activity. The efficient inducers of NADH-quinone reductase in E. coli contain 1,4-quinone structure as a part of the inductive signal. These compounds belong to Michael acceptors and are likely to conjugate with thiol compounds such as glutathione. |
| Links | |
| Keywords |
Benzoquinones/pharmacology; Enzyme Induction/drug effects; Escherichia coli/drug effects; Escherichia coli/enzymology; FMN Reductase; NAD(P)H Dehydrogenase (Quinone)/biosynthesis; NADH, NADPH Oxidoreductases/biosynthesis; Naphthoquinones/pharmacology; Superoxide Dismutase/biosynthesis; Vitamin K/pharmacology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.