PMID:15383285
| Citation |
Folta-Stogniew, E, O'Malley, S, Gupta, R, Anderson, KS and Radding, CM (2004) Exchange of DNA base pairs that coincides with recognition of homology promoted by E. coli RecA protein. Mol. Cell 15:965-75 |
|---|---|
| Abstract |
The unresolved mechanism by which a single strand of DNA recognizes homology in duplex DNA is central to understanding genetic recombination and repair of double-strand breaks. Using stopped-flow fluorescence we monitored strand exchange catalyzed by E. coli RecA protein, measuring simultaneously the rate of exchange of A:T base pairs and the rates of formation and dissociation of the three-stranded intermediates called synaptic complexes. The rate of exchange of A:T base pairs was indistinguishable from the rate of formation of synaptic complexes, whereas the rate of displacement of a single strand from complexes was five to ten times slower. This physical evidence shows that a subset of bases exchanges at a rate that is fast enough to account for recognition of homology. Together, several studies suggest that a mechanism governed by the dynamic structure of DNA and catalyzed by diverse enzymes underlies both recognition of homology and initiation of strand exchange. |
| Links |
PubMed Online version:10.1016/j.molcel.2004.08.017 |
| Keywords |
Base Composition; Base Pairing; Base Sequence; DNA, Bacterial/chemistry; DNA, Bacterial/metabolism; DNA, Single-Stranded/metabolism; Escherichia coli Proteins/metabolism; Kinetics; Rec A Recombinases/metabolism; Sequence Homology, Nucleic Acid |
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