PMID:15381101
| Citation |
Sonnleitner, E, Napetschnig, J, Afonyushkin, T, Ecker, K, Vecerek, B, Moll, I, Kaberdin, VR and Bläsi, U (2004) Functional effects of variants of the RNA chaperone Hfq. Biochem. Biophys. Res. Commun. 323:1017-23 |
|---|---|
| Abstract |
The ring-shaped RNA chaperone Hfq has recently received much attention owing to its multiple roles in RNA metabolism. In this study we have performed a mutational analysis of the Escherichia coli hfq gene, and have studied the effects of amino acid substitutions at several positions in the Hfq protein as well as of C-terminal truncations on its role in phage Qbeta replication, in repression of a target mRNA, and on the stability of the small regulatory RNA DsrA. These functional studies provided insights into the interaction of Hfq with RNA and suggested a role for the C-terminus of Hfq in DsrA stability. |
| Links |
PubMed Online version:10.1016/j.bbrc.2004.08.190 |
| Keywords |
Allolevivirus/growth & development; Amino Acid Substitution; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/virology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Host Factor 1 Protein/genetics; Host Factor 1 Protein/metabolism; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Mutagenesis, Site-Directed; RNA/genetics; RNA/metabolism; RNA, Small Untranslated; RNA, Untranslated/genetics; RNA, Untranslated/metabolism; Recombinant Proteins/metabolism; Structure-Activity Relationship |
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