PMID:15377268
| Citation |
Vasilieva, SV, Moshkovskaya, EY, Sanina, NA, Aldoshin, SM and Vanin, AF (2004) Genetic signal transduction by nitrosyl-iron complexes in Escherichia coli. Biochemistry Mosc. 69:883-9 |
|---|---|
| Abstract |
Nitrosyl-iron complexes used as aqueous preparations of binuclear dinitrosyl-iron complex with glutathione (DNICglu), initially polycrystalline preparations of binuclear tetranitrosyl-iron complex with thiosulfate (TNICthio), and also binuclear tetranitrosyl-iron complex with aminotriazole (TNICatria) and mononuclear dinitrosyl-iron complex with triazole (DNICtria) in the concentration to 0.1 mM activated expression of the soxS and sfiA genes in Escherichia coli. Higher concentrations of polycrystalline preparations of low stability in aqueous solutions were cytotoxic, whereas DNICglu, which is more stable in water (up to two days), increased the gene expression on increase in its concentration to 0.5 mM. The iron chelating agent o-phenanthroline completely inhibited the gene expression induced by all compounds studied. The genetic signal transduction seemed to be realized not by nitric oxide molecules and/or iron ions released in solutions but directly by the complexes themselves, which activate transcriptional proteins by transfer onto them of nitrosyl-iron groups [Fe+(NO+)2]. |
| Links | |
| Keywords |
Electron Spin Resonance Spectroscopy; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Gene Expression Regulation, Bacterial/drug effects; Iron/chemistry; Iron/metabolism; Iron/pharmacology; Iron Chelating Agents/pharmacology; Molecular Structure; Nitrogen Oxides/chemistry; Nitrogen Oxides/metabolism; Nitrogen Oxides/pharmacology; Phenanthrolines/pharmacology; SOS Response (Genetics)/drug effects; Solutions/chemistry; Trans-Activators/genetics; Water/chemistry |
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