PMID:15375373
| Citation |
Houben, EN, ten Hagen-Jongman, CM, Brunner, J, Oudega, B and Luirink, J (2004) The two membrane segments of leader peptidase partition one by one into the lipid bilayer via a Sec/YidC interface. EMBO Rep. 5:970-5 |
|---|---|
| Abstract |
We have addressed the mechanism of insertion of both transmembrane segments (TMs) of leader peptidase, a double-spanning protein, into the Escherichia coli inner membrane. Using photo-crosslinking, the first TM (H1) was shown to insert at a Sec-translocon/YidC interface in a fixed orientation. H1 lost its contacts with the Sec-translocon and gained access to lipids near YidC soon after complete exposure outside the ribosome. Following lipid integration, it moved away from the Sec/YidC insertion site. The second TM (H2) inserted and interacted with SecY and YidC in a similar transient fashion. The data are consistent with a linear integration model in which the TMs of polytopic inner membrane proteins move one by one from a Sec/YidC insertion site into the lipid bilayer. We propose that YidC assists the lipid partitioning of single TMs. |
| Links |
PubMed PMC1299158 Online version:10.1038/sj.embor.7400261 |
| Keywords |
Adenosine Triphosphatases/metabolism; Bacterial Proteins/metabolism; Cell Membrane/metabolism; Escherichia coli/enzymology; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Lipid Bilayers/metabolism; Membrane Proteins/metabolism; Membrane Transport Proteins/metabolism; Protein Structure, Tertiary; Serine Endopeptidases/metabolism |
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