PMID:15372038
| Citation |
Vetsch, M, Puorger, C, Spirig, T, Grauschopf, U, Weber-Ban, EU and Glockshuber, R (2004) Pilus chaperones represent a new type of protein-folding catalyst. Nature 431:329-33 |
|---|---|
| Abstract |
Adhesive type 1 pili from uropathogenic Escherichia coli strains have a crucial role during infection by mediating the attachment to and potentially the invasion of host tissue. These filamentous, highly oligomeric protein complexes are assembled by the 'chaperone-usher' pathway, in which the individual pilus subunits fold in the bacterial periplasm and form stoichiometric complexes with a periplasmic chaperone molecule that is essential for pilus assembly. The chaperone subsequently delivers the subunits to an assembly platform (usher) in the outer membrane, which mediates subunit assembly and translocation to the cell surface. Here we show that the periplasmic type 1 pilus chaperone FimC binds non-native pilus subunits and accelerates folding of the subunit FimG by 100-fold. Moreover, we find that the FimC-FimG complex is formed quantitatively and very rapidly when folding of FimG is initiated in the presence of both FimC and the assembly-competent subunit FimF, even though the FimC-FimG complex is thermodynamically less stable than the FimF-FimG complex. FimC thus represents a previously unknown type of protein-folding catalyst, and simultaneously acts as a kinetic trap preventing spontaneous subunit assembly in the periplasm. |
| Links |
PubMed Online version:10.1038/nature02891 |
| Keywords |
Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Catalysis; Escherichia coli/chemistry; Escherichia coli/metabolism; Escherichia coli/pathogenicity; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Fimbriae Proteins/chemistry; Fimbriae Proteins/metabolism; Fimbriae, Bacterial/chemistry; Fimbriae, Bacterial/metabolism; Kinetics; Macromolecular Substances; Molecular Chaperones/metabolism; Periplasm/chemistry; Periplasm/metabolism; Protein Folding; Protein Subunits/chemistry; Protein Subunits/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
