PMID:15351721
| Citation |
Mashimo, K, Nagata, Y, Kawata, M, Iwasaki, H and Yamamoto, K (2004) Role of the RuvAB protein in avoiding spontaneous formation of deletion mutations in the Escherichia coli K-12 endogenous tonB gene. Biochem. Biophys. Res. Commun. 323:197-203 |
|---|---|
| Abstract |
The endogenous tonB gene of Escherichia coli was used as a target for spontaneous deletion mutations which were isolated from ruvAB-, recG-, and ruvC- cells. The rates of tonB mutation were essentially the same in ruv+, ruvAB-, recG-, and ruvC- cells. We analyzed tonB mutants by sequencing. In the ruv+, recG-, and ruvC- strains, the spectra were different from those obtained from the ruvAB- cells, where deletions dominated followed by IS insertions, base substitutions, and frameshifts, in that order. We then analyzed the tonB-trp large deletion, due to simultaneous mutations of the trp operon, and found that the frequency in ruvAB- was higher than those in ruv+, recG-, and ruvC- cells. To characterize deletion formation further, we analyzed all the tonB mutants from one colicin plate. Seven deletions were identified at five sites from the 45 tonB mutants of ruv+ cells and 24 deletions at 11 sites from the 43 tonB mutants of ruvAB- cells. Thus, the ruvAB- strain is a deletion mutator. We discuss the role of RuvAB in avoiding deletions. |
| Links |
PubMed Online version:10.1016/j.bbrc.2004.08.078 |
| Keywords |
Alleles; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Colicins/metabolism; DNA Helicases/genetics; DNA Helicases/physiology; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Endodeoxyribonucleases/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Gene Deletion; Membrane Proteins/genetics; Models, Genetic; Mutagenesis; Mutation; Plasmids/metabolism |
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