PMID:1534062
| Citation |
Autexier, C and DuBow, MS (1992) The Escherichia coli Mu/D108 phage ner homologue gene (nlp) is transcribed and evolutionarily conserved among the Enterobacteriaceae. Gene 114:13-8 |
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| Abstract |
The Escherichia coli nlp gene is highly homologous to the regulatory ner genes of transposable coliphages, Mu and D108. It was discovered, via its action when overexpressed, as a positive activator of mal gene expression in a cya- crp*1 strain. Chromosomal disruption of the nlp gene by insertion of a promoterless luxAB reporter gene revealed that nlp is nonessential for E. coli viability. Light measurements from the resulting nlp::luxAB transcriptional fusion, plus RNA dot blot analysis, suggest that nlp is transcribed. Southern-blot analyses of DNAs from several bacterial species were performed and indicate that nlp is evolutionarily conserved, but only among closely related Enterobacteriaceae. |
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| Keywords |
Bacteriophage mu/genetics; Blotting, Southern; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Fermentation; Genes, Bacterial/genetics; Genes, Regulator/genetics; Genes, Viral; Recombinant Fusion Proteins/genetics; Repressor Proteins/genetics; Sequence Homology, Nucleic Acid; Transcription, Genetic/genetics; Viral Proteins/genetics; Viral Regulatory and Accessory Proteins |
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