PMID:15333942
| Citation |
Nakamura, T, Doi, T, Sekiguchi, M and Yamagata, Y (2004) Crystallization and preliminary X-ray analysis of Escherichia coli MutT in binary and ternary complex forms. Acta Crystallogr. D Biol. Crystallogr. 60:1641-3 |
|---|---|
| Abstract |
During replication, Escherichia coli MutT prevents the misincorporation of mutagenic 8-oxoguanine into nascent DNA strands opposite adenine by hydrolyzing 8-oxo-dGTP in nucleotide pools to 8-oxo-dGMP. E. coli MutT is the most widely investigated member of the Nudix hydrolase family, which is large and found in all organisms. By co-crystallization of MutT with 8-oxo-dGMP, a reaction product, crystals of the binary complex were obtained using ammonium sulfate as a precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 37.9, b = 56.0, c = 59.4 A. Assuming the presence of one protein-nucleotide complex in the asymmetric unit, the Matthews coefficient V(M) is 2.1 A(3) Da(-1). Crystals of the ternary complex were prepared by soaking crystals of the binary complex in 1 mM MnCl(2) solution. They diffracted to 1.96 and 2.56 A resolution, respectively. |
| Links |
PubMed Online version:10.1107/S0907444904016403 |
| Keywords |
Crystallization; Data Collection; Deoxyguanine Nucleotides; Escherichia coli/chemistry; Escherichia coli Proteins/chemistry; Phosphoric Monoester Hydrolases/chemistry; Pyrophosphatases; X-Ray Diffraction |
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