PMID:15333933
| Citation |
Vassylyeva, MN, Perederina, AA, Svetlov, V, Yokoyama, S, Artsimovitch, I and Vassylyev, DG (2004) Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription factor DksA from Escherichia coli. Acta Crystallogr. D Biol. Crystallogr. 60:1611-3 |
|---|---|
| Abstract |
The Escherichia coli gene encoding a regulator of stringent response and virulence, DksA, which contains a canonical Zn finger motif, was cloned and expressed, and the purified protein was crystallized by the hanging-drop vapor-diffusion technique in two different space groups, P2(1)2(1)2(1) (a = 91.32, b = 96.59, c = 117.48 A) and C222 (a = 80.6, b = 115.1, c = 149.57 A). The crystals belonging to space group P2(1)2(1)2(1), improved by macroseeding, diffract beyond 2.0 A at a synchrotron. Three complete atomic resolution multiple anomalous dispersion diffraction data sets were collected from the same crystal of the P2(1)2(1)2(1) crystal form at the absorption edge for Zn atoms. |
| Links |
PubMed Online version:10.1107/S0907444904015033 |
| Keywords |
Cloning, Molecular; Crystallization; Crystallography, X-Ray; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/isolation & purification; Models, Molecular; Reverse Transcriptase Polymerase Chain Reaction; Synchrotrons; Zinc Fingers |
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