PMID:15330853
| Citation |
Ogino, H, Wachi, M, Ishii, A, Iwai, N, Nishida, T, Yamada, S, Nagai, K and Sugai, M (2004) FtsZ-dependent localization of GroEL protein at possible division sites. Genes Cells 9:765-71 |
|---|---|
| Abstract |
When Escherichia coli is treated with penicillin, the envelopes bulge at the centre of the cells and the cells then lyse. The bulges expand into vesicle-like structures termed penicillin-induced vesicles. We have developed a method to isolate these structures and have shown that they contain mainly membrane proteins plus a high concentration of a 60 kDa protein. The N-terminal amino acid sequence of the protein is identical to that of GroEL protein. Western blotting analysis using anti-GroEL antibody showed that GroEL is indeed concentrated in the vesicles. Indirect immuno-fluorescence microscopy showed that GroEL protein is localized at the centre of the cells at the site of formation of FtsZ-rings. Localization of GroEL is dependent on FtsZ but not other Fts proteins. GroEL mutants formed elongated cells having no or asymmetrically localized FtsZ-rings at the restrictive temperature. These findings suggest a possible role of the GroEL protein in cell division. |
| Links |
PubMed Online version:10.1111/j.1365-2443.2004.00770.x |
| Keywords |
Cell Division; Chaperonin 60/analysis; Chaperonin 60/physiology; Cytoplasmic Vesicles/chemistry; Cytoplasmic Vesicles/ultrastructure; Escherichia coli/chemistry; Escherichia coli/cytology; Escherichia coli/ultrastructure; Escherichia coli Proteins/physiology; Microscopy, Fluorescence; Penicillins/pharmacology |
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