PMID:1530848
| Citation |
Groisman, EA, Heffron, F and Solomon, F (1992) Molecular genetic analysis of the Escherichia coli phoP locus. J. Bacteriol. 174:486-91 |
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| Abstract |
We have cloned the Escherichia coli phoP gene, a member of the family of environmentally responsive two-component systems, and found its deduced amino acid sequence to be 93% identical to that of the Salmonella typhimurium homolog, which encodes a major virulence regulator necessary for intramacrophage survival and resistance to cationic peptides of phagocytic cells. The phoP gene was mapped to kilobase 1202 on the Kohara map (25-min region) of the E. coli genome (Y. Kohara, K. Akiyama, and K. Isono, Cell 50:495-508, 1987) and found to be transcribed in a counterclockwise direction. Both E. coli and S. typhimurium phoP mutants were more sensitive than their isogenic wild-type strains to the frog-derived antibacterial peptide magainin 2, suggesting a role for PhoP in the response to various stresses in both enteric species. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacteriophage mu/genetics; Base Sequence; Cloning, Molecular; DNA, Bacterial/chemistry; Escherichia coli/genetics; Escherichia coli/physiology; Genes, Bacterial; Molecular Sequence Data; Mutation; Phenotype; Replicon; Restriction Mapping; Sequence Homology, Nucleic Acid; Transcription Factors/genetics |
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