PMID:1530607
| Citation |
Whittaker, JJ and Jackson, JH (1992) Maintenance of repression control of the ilvGMEDA operon in a temperature-sensitive leucyl-transfer RNA synthetase mutant of Escherichia coli K-12 at a restrictive temperature. Biochem. Biophys. Res. Commun. 187:1106-12 |
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| Abstract |
The mechanism of L-leucine regulation of ilvGMEDA is thought to be by ribosome-mediated attenuation that is dependent upon the concentration of Leu-tRNA(Leu) which results from leucyl-tRNA synthetase (LeuRS) activity. The requirement for LeuRS activity in attenuation control was tested in an Escherichia coli K-12 strain containing a temperature-sensitive LeuRS and the ilvGMEDA operon with an active ilvGM. Growth of this strain at 30 degrees C followed by a shift to 37 degrees C to inactivate the LeuRS revealed that ilvGM expression decreased at the restrictive temperature whereas the downstream gene expression was slightly elevated. We suggest that ilvGM does not respond to a deattenuation signal, and that, possibly, a secondary repression/derepression mechanism exists. |
| Links | |
| Keywords |
Amino Acid Sequence; Codon; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Leucine/genetics; Leucine/pharmacology; Leucine-tRNA Ligase/chemistry; Leucine-tRNA Ligase/genetics; Molecular Sequence Data; Mutation; Operon; Protein Sorting Signals/chemistry; Protein Sorting Signals/genetics; RNA, Transfer, Amino Acyl/metabolism; Temperature |
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