PMID:15297874
| Citation |
Owens, RM, Pritchard, G, Skipp, P, Hodey, M, Connell, SR, Nierhaus, KH and O'Connor, CD (2004) A dedicated translation factor controls the synthesis of the global regulator Fis. EMBO J. 23:3375-85 |
|---|---|
| Abstract |
BipA is a highly conserved protein with global regulatory properties in Escherichia coli. We show here that it functions as a translation factor that is required specifically for the expression of the transcriptional modulator Fis. BipA binds to ribosomes at a site that coincides with that of elongation factor G and has a GTPase activity that is sensitive to high GDP:GTP ratios and stimulated by 70S ribosomes programmed with mRNA and aminoacylated tRNAs. The growth rate-dependent induction of BipA allows the efficient expression of Fis, thereby modulating a range of downstream processes, including DNA metabolism and type III secretion. We propose a model in which BipA destabilizes unusually strong interactions between the 5' untranslated region of fis mRNA and the ribosome. Since BipA spans phylogenetic domains, transcript-selective translational control for the 'fast-track' expression of specific mRNAs may have wider significance. |
| Links |
PubMed PMC514516 Online version:10.1038/sj.emboj.7600343 |
| Keywords |
5' Untranslated Regions/genetics; Base Sequence; Cell Proliferation; Enzyme Inhibitors/pharmacology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins/antagonists & inhibitors; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Factor For Inversion Stimulation Protein; GTP Phosphohydrolases/antagonists & inhibitors; GTP Phosphohydrolases/genetics; GTP Phosphohydrolases/metabolism; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Molecular Weight; Nucleic Acid Conformation; Phosphoproteins/antagonists & inhibitors; Phosphoproteins/genetics; Phosphoproteins/metabolism; Protein Binding; Protein Biosynthesis; RNA, Messenger/chemistry; RNA, Messenger/genetics; Ribosomes/chemistry; Ribosomes/metabolism; Transcription Factors/biosynthesis; Transcription Factors/genetics |
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