PMID:15278785
| Citation |
Ollagnier-de-Choudens, S, Sanakis, Y and Fontecave, M (2004) SufA/IscA: reactivity studies of a class of scaffold proteins involved in [Fe-S] cluster assembly. J. Biol. Inorg. Chem. 9:828-38 |
|---|---|
| Abstract |
IscA/SufA proteins belong to complex protein machineries which are involved in iron-sulfur cluster biosynthesis. They are defined as scaffold proteins from which preassembled clusters are transferred to target apoproteins. The experiments described here demonstrate that the transfer reaction proceeds in two observable steps: a first fast one leading to a protein-protein complex between the cluster donor (SufA/IscA) and the acceptor (biotin synthase), and a slow one consisting of cluster transfer leading to the apoform of the scaffold protein and the holoform of the target protein. Mutation of cysteines in the acceptor protein specifically inhibits the second step of the reaction, showing that these cysteines are involved in the cluster transfer mechanism but not in complex formation. No cluster transfer from IscA to IscU, another scaffold of the isc operon, could be observed, whereas IscU was shown to be an efficient cluster source for cluster assembly in IscA. Implications of these results are discussed. |
| Links |
PubMed Online version:10.1007/s00775-004-0581-9 |
| Keywords |
Apoproteins/chemistry; Apoproteins/metabolism; Carrier Proteins/chemistry; Carrier Proteins/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Iron/chemistry; Iron-Sulfur Proteins/chemistry; Iron-Sulfur Proteins/metabolism; Spectrum Analysis; Sulfur/chemistry; Sulfurtransferases/chemistry; Sulfurtransferases/metabolism; Time Factors |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.