PMID:15277681
| Citation |
von Ballmoos, C, Brunner, J and Dimroth, P (2004) The ion channel of F-ATP synthase is the target of toxic organotin compounds. Proc. Natl. Acad. Sci. U.S.A. 101:11239-44 |
|---|---|
| Abstract |
ATP is the universal energy currency of living cells, and the majority of it is synthesized by the F1F0 ATP synthase. Inhibitors of this enzyme are therefore potentially detrimental for all life forms. Tributyltin chloride (TBT-Cl) inhibits ATP hydrolysis by the Na(+)-translocating ATP synthase of Ilyobacter tartaricus or the H(+)-translocating counterpart of Escherichia coli with apparent Ki of 200 nM. To target the site of this inhibition, we synthesized a tritium-labeled derivative of TBT-Cl in which one of the butyl groups was replaced by a photoactivatable aryldiazirine residue. Upon illumination, subunit a of the ATP synthase becomes specifically modified, and this labeling is suppressed in the presence of the original inhibitor. In case of the Na+ ATP synthase, labeling is also suppressed in the presence of Na+ ions, suggesting an interference in Na+ or TBT-Cl binding to subunit a. This interference is corroborated by the protection of ATP hydrolysis from TBT-Cl inhibition by 105 mM Na+. TBT-Cl strongly inhibits Na+ exchange by the reconstituted I. tartaricus ATP synthase. Taken together these results indicate that the subunit a ion channel is the target site for ATPase inhibition by toxic organotin compounds. An inhibitor interacting specifically with this site has not been reported previously. |
| Links |
PubMed PMC509188 Online version:10.1073/pnas.0402869101 |
| Keywords |
Adenosine Triphosphatases/chemistry; Adenosine Triphosphatases/metabolism; Adenosine Triphosphate/metabolism; Binding Sites/drug effects; Cation Transport Proteins/chemistry; Cation Transport Proteins/metabolism; Cell Membrane/enzymology; Enzyme Inhibitors/chemical synthesis; Escherichia coli; Mitochondrial Proton-Translocating ATPases/chemistry; Mitochondrial Proton-Translocating ATPases/metabolism; Organotin Compounds/chemical synthesis; Organotin Compounds/toxicity; Photoaffinity Labels; Photochemistry; Proteolipids/metabolism; Sodium/pharmacokinetics; Solubility; Trialkyltin Compounds/chemistry |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.