PMID:15268940

Citation	Koyanagi, T, Katayama, T, Suzuki, H and Kumagai, H (2004) The LIV-I/LS system as a determinant of azaserine sensitivity of Escherichia coli K-12. FEMS Microbiol. Lett. 237:73-7
Abstract	The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been elucidated except that all the azaserine-resistant strains isolated so far carry lesions in the aroP gene as a primary determinant. Here, we demonstrate that, in addition to AroP, the LIV-I/LS system, an ATP-binding cassette type transporter, is involved in azaserine sensitivity of E. coli, by genetic analysis and transport studies, in which Ki value for azaserine was determined to be approximately 10(-3) M.
Links	PubMed Online version:10.1016/j.femsle.2004.06.017
Keywords	Amino Acid Transport Systems/genetics; Amino Acid Transport Systems/metabolism; Anti-Bacterial Agents/metabolism; Anti-Bacterial Agents/pharmacology; Azaserine/metabolism; Azaserine/pharmacology; Escherichia coli/drug effects; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Genes, Bacterial; Genetic Complementation Test; Leucine/metabolism; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Microbial Sensitivity Tests; Phenylalanine/metabolism
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