PMID:15247236
| Citation |
Vergnes, A, Gouffi-Belhabich, K, Blasco, F, Giordano, G and Magalon, A (2004) Involvement of the molybdenum cofactor biosynthetic machinery in the maturation of the Escherichia coli nitrate reductase A. J. Biol. Chem. 279:41398-403 |
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| Abstract |
The maturation of Escherichia coli nitrate reductase A requires the incorporation of the Mo-(bis-MGD) cofactor to the apoprotein. For this process, the NarJ chaperone is strictly required. We report the first description of protein interactions between molybdenum cofactor biosynthetic proteins (MogA, MoeA, MobA, and MobB) and the aponitrate reductase (NarG) using a bacterial two-hybrid approach. Two conditions have to be satisfied to allow the visualization of the interactions, (i) the presence of an active and mature molybdenum cofactor and (ii) the presence of the NarJ chaperone and of the NarG structural partner subunit, NarH. Formation of tungsten-substituted cofactor prevents the interaction between NarG and the four biosynthetic proteins. Our results suggested that the final stages of molybdenum cofactor biosynthesis occur on a complex made up by MogA, MoeA, MobA, and MobB, which is also in charge with the delivery of the mature cofactor onto the aponitrate reductase A in a NarJ-assisted process. |
| Links |
PubMed Online version:10.1074/jbc.M407087200 |
| Keywords |
Apoproteins/metabolism; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/metabolism; Macromolecular Substances; Molecular Chaperones/metabolism; Molybdenum; Nitrate Reductase; Nitrate Reductases/biosynthesis; Protein Subunits/metabolism; Sulfurtransferases/metabolism; Trans-Activators/metabolism; Two-Hybrid System Techniques |
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