PMID:15215612
| Citation |
Tanaka, T, Ando, T, Haga, S and Kikuchi, Y (2004) Examining the bases of the J3/4 domain of Escherichia coli ribonuclease P. Biosci. Biotechnol. Biochem. 68:1388-92 |
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| Abstract |
We prepared several mutants of the J3/4 and P4 domains of Escherichia coli ribonuclease P (RNase P): A62G, A62U, G63C/G64C, A65G, A67G, U69A, U69G, U69C, U69Delta, and U69UU. Comparison of the ribozyme and holo enzyme reactions at various concentrations of magnesium ions showed that the presence of a bulge at U69 in the P4 domain was important in the holo enzyme. The results also showed that the conserved bases G63 and G64 in the J3/4 domain were important for efficient ribozyme reactions but were replaceable in the presence of the protein component. Our data showed that the bases in the J3/4 and P4 domains displayed different responses to the metal ions that were affected by the presence of the protein component. |
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| Keywords |
Base Sequence; Escherichia coli Proteins/chemistry; Magnesium; Mutagenesis, Site-Directed; Mutation, Missense; Nucleic Acid Conformation; Protein Structure, Tertiary; RNA, Catalytic/metabolism; Ribonuclease P/chemistry; Ribonuclease P/genetics; Ribonuclease P/metabolism |
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