PMID:15209514
| Citation |
Greenberg, MM, Weledji, YN, Kim, J and Bales, BC (2004) Repair of oxidized abasic sites by exonuclease III, endonuclease IV, and endonuclease III. Biochemistry 43:8178-83 |
|---|---|
| Abstract |
2-Deoxyribonolactone (L) and the C4'-oxidized abasic site (C4-AP) are produced by a variety of DNA-damaging agents. If not repaired, these lesions can be mutagenic. Exonuclease III and endonuclease IV are the major enzymes in E. coli responsible for 5'-incision of abasic sites (APs), the first steps in AP repair. Endonuclease III efficiently excises AP lesions via intermediate Schiff-base formation. Incision of L and C4-AP lesions by exonuclease III and endonuclease IV was determined under steady-state conditions using oligonucleotide duplexes containing the lesions at defined sites. An abasic lesion (AP) in an otherwise identical DNA sequence was incised by exonuclease III or endonuclease IV approximately 6-fold more efficiently than either of the oxidized abasic sites (L, C4-AP). Endonuclease IV incision efficiency of 2-deoxyribonolactone or C4-AP was independent of whether the lesion was opposite dA or dG. 2-Deoxyribonolactone is known to cross-link to endonuclease III (Hashimoto, M. (2001) J. Am. Chem. Soc. 123, 3161.). However, the C4-AP lesion is efficiently excised by endonuclease III. Oxidized abasic site repair by endonuclease IV and endonuclease III (C4-AP only) is approximately 100-fold less efficient than repair by exonuclease III. These results suggest that the first step of C4-AP and L oxidized abasic site repair will be the same as that of regular AP lesions in E. coli. |
| Links |
PubMed Online version:10.1021/bi0496236 |
| Keywords |
Base Pairing; Base Sequence; Binding Sites; DNA Damage/drug effects; DNA Damage/genetics; DNA Damage/radiation effects; DNA Repair; Deoxyribonuclease IV (Phage T4-Induced)/chemistry; Deoxyribonuclease IV (Phage T4-Induced)/metabolism; Endodeoxyribonucleases/chemistry; Endodeoxyribonucleases/metabolism; Escherichia coli/enzymology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Exodeoxyribonucleases/chemistry; Exodeoxyribonucleases/metabolism; Kinetics; Molecular Structure; Oligonucleotides/genetics; Oxidation-Reduction; Substrate Specificity; Sugar Acids/chemistry; Sugar Acids/metabolism; Sugar Acids/radiation effects |
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