PMID:1520738
| Citation |
Nakamura, Y and Kawakami, K (1992) Overproduction and purification of lysyl-tRNA synthetase encoded by the herC gene of E coli. Biochimie 74:581-4 |
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| Abstract |
Lysyl-tRNA synthetases are synthesized from two distinct genes in E coli, lysS and lysU, but neither gene product has been purified distinctively by using overproducing systems. The lysS gene has been identified by a herC mutation which restores maintenance of the mutant ColE1 replicon. The herC gene product was overproduced by using a tac promoter fusion and purified to homogeneity. The purified HerC protein possesses a lysyl-tRNA synthetase activity as predicted by the sequence identity of herC to lysS. The procedure is useful for rapid mass-scale purification of lysyl-tRNA synthetase. |
| Links | |
| Keywords |
Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Lysine-tRNA Ligase/biosynthesis; Lysine-tRNA Ligase/isolation & purification; Plasmids; Recombinant Proteins/biosynthesis; Recombinant Proteins/isolation & purification |
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