PMID:15205445

Citation	Kamionka, A, Sehnal, M, Scholz, O and Hillen, W (2004) Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants. J. Bacteriol. 186:4399-401
Abstract	We report a regulation system in Escherichia coli for independent regulation of two distinct reporter genes by application of Tet repressors with different specificities. One Tet repressor variant comprises wild-type tet operator (tetO) recognition and exclusive induction with the novel inducer 4-dedimethylamino-anhydrotetracycline. The other Tet repressor variant shows tetO-4C recognition and induction with tetracycline. We demonstrate that both variants are independently active in vivo and allow selective regulation of two genes in the same cell without any cross talk.
Links	PubMed PMC421600 Online version:10.1128/JB.186.13.4399-4401.2004
Keywords	Endo-1,4-beta Xylanases/genetics; Escherichia coli/genetics; Escherichia coli Proteins/physiology; Gene Expression Regulation, Bacterial; Tetracycline/pharmacology; beta-Glucosidase/genetics
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