PMID:15194690
| Citation |
Wang, H and Cronan, JE (2004) Functional replacement of the FabA and FabB proteins of Escherichia coli fatty acid synthesis by Enterococcus faecalis FabZ and FabF homologues. J. Biol. Chem. 279:34489-95 |
|---|---|
| Abstract |
The anaerobic unsaturated fatty acid synthetic pathway of Escherichia coli requires two specialized proteins, FabA and FabB. However, the fabA and fabB genes are found only in the Gram-negative alpha- and gamma-proteobacteria, and thus other anaerobic bacteria must synthesize these acids using different enzymes. We report that the Gram-positive bacterium Enterococcus faecalis encodes a protein, annotated as FabZ1, that functionally replaces the E. coli FabA protein, although the sequence of this protein aligns much more closely with E. coli FabZ, a protein that plays no specific role in unsaturated fatty acid synthesis. Therefore E. faecalis FabZ1 is a bifunctional dehydratase/isomerase, an enzyme activity heretofore confined to a group of Gram-negative bacteria. The FabZ2 protein is unable to replace the function of E. coli FabZ, although FabZ2, a second E. faecalis FabZ homologue, has this ability. Moreover, an E. faecalis FabF homologue (FabF1) was found to replace the function of E. coli FabB, whereas a second FabF homologue was inactive. From these data it is clear that bacterial fatty acid biosynthetic pathways cannot be deduced solely by sequence comparisons. |
| Links |
PubMed Online version:10.1074/jbc.M403874200 |
| Keywords |
3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/genetics; 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/physiology; Acyl Carrier Protein/chemistry; Arabinose/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Cell-Free System; DNA/metabolism; Enterococcus faecalis/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins; Fatty Acid Synthetase Complex, Type II; Fatty Acids/biosynthesis; Fatty Acids/chemistry; Genetic Complementation Test; Hydro-Lyases/genetics; Hydro-Lyases/physiology; Multienzyme Complexes/genetics; Mutation; Phospholipids/metabolism; Plasmids/metabolism |
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