PMID:15167889
| Citation |
Grompone, G, Ehrlich, D and Michel, B (2004) Cells defective for replication restart undergo replication fork reversal. EMBO Rep. 5:607-12 |
|---|---|
| Abstract |
We have studied the fate of blocked replication forks with the use of the Escherichia coli priA mutant, in which spontaneously arrested replication forks persist owing to the lack of the major replication restart pathway. Such blocked forks undergo a specific reaction named replication fork reversal, in which newly synthesized strands anneal to form a DNA double-strand end adjacent to a four-way junction. Indeed, (i) priA recB mutant chromosomes are linearized by a reaction that requires the presence of the Holliday junction resolvase RuvABC, and (ii) RuvABC-dependent linearization is prevented by the presence of RecBC. Replication fork reversal in a priA mutant occurs independently of the recombination proteins RecA and RecR. recBC inactivation does not affect priA mutant viability but prevents priA chronic SOS induction. We propose that, in the absence of PriA, RecBC action at reversed forks does not allow replication restart, which leads to the accumulation of SOS-inducing RecA filaments. Our results suggest that types of replication blockage that cause replication fork reversal occur spontaneously. |
| Links |
PubMed PMC1299077 Online version:10.1038/sj.embor.7400167 |
| Keywords |
Adenosine Triphosphatases/deficiency; Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Bacterial Proteins/metabolism; DNA/metabolism; DNA Helicases/deficiency; DNA Helicases/genetics; DNA Helicases/metabolism; DNA Replication/genetics; DNA Replication/physiology; DNA, Cruciform/metabolism; DNA-Binding Proteins/metabolism; Endodeoxyribonucleases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Exodeoxyribonuclease V/genetics; Exodeoxyribonuclease V/metabolism; Holliday Junction Resolvases/metabolism; Mutation; SOS Response (Genetics)/genetics; SOS Response (Genetics)/physiology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.