PMID:15159580
| Citation |
Teartasin, W, Limpkin, C, Glod, F, Spencer, J, Cox, RJ, Simpson, TJ, Crosby, J, Crump, MP and Hadfield, AT (2004) Expression, purification and preliminary X-ray diffraction analysis of a ketoreductase from a type II polyketide synthase. Acta Crystallogr. D Biol. Crystallogr. 60:1137-8 |
|---|---|
| Abstract |
Polyketide metabolites produced by bacteria and other organisms include antibiotics, anticancer and antifungal compounds. In type II polyketide synthesis, three enzymes are sufficient to form a polyketide product of the requisite chain length, although the fidelity of the first cyclization is variable. Addition of ketoreductase (KR) to this system results in the formation of a product with correct cyclization and reduction. This paper reports the cloning of the Streptomyces coelicolor actIII ORF5 gene that codes for the ketoreductase. The 261-amino-acid protein has been overexpressed with a 20-residue His tag, purified by affinity chromatography and crystallized in space group P3(2)21, with unit-cell parameters a = b = 103.9, c = 123.1 angstroms. The crystals diffract to 2.5 angstroms resolution. A complete data set has been collected and structure solution and refinement is under way. |
| Links |
PubMed Online version:10.1107/S0907444904007905 |
| Keywords |
Alcohol Oxidoreductases/chemistry; Alcohol Oxidoreductases/genetics; Alcohol Oxidoreductases/metabolism; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Histidine/chemistry; Hydrogen-Ion Concentration; Open Reading Frames; Polyketide Synthases/chemistry; Protein Conformation; Streptomyces coelicolor/genetics; Streptomyces coelicolor/metabolism; X-Ray Diffraction/methods |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.