PMID:15138263
| Citation |
Lusetti, SL, Voloshin, ON, Inman, RB, Camerini-Otero, RD and Cox, MM (2004) The DinI protein stabilizes RecA protein filaments. J. Biol. Chem. 279:30037-46 |
|---|---|
| Abstract |
When DinI is present at concentrations that are stoichiometric with those of RecA or somewhat greater, DinI has a substantial stabilizing effect on RecA filaments bound to DNA. Exchange of RecA between free and bound forms was almost entirely suppressed, and highly stable filaments were documented with several different experimental methods. DinI-mediated stabilization did not affect RecA-mediated ATP hydrolysis and LexA co-protease activities. Initiation of DNA strand exchange was affected in a DNA structure-dependent manner, whereas ongoing strand exchange was not affected. Destabilization of RecA filaments occurred as reported in earlier work but only when DinI protein was present at very high concentrations, generally superstoichiometric, relative to the RecA protein concentration. DinI did not facilitate RecA filament formation but stabilized the filaments only after they were formed. The interaction between the RecA protein and DinI was modulated by the C terminus of RecA. We discuss these results in the context of a new hypothesis for the role of DinI in the regulation of recombination and the SOS response. |
| Links |
PubMed Online version:10.1074/jbc.M403064200 |
| Keywords |
Adenosine Triphosphate/chemistry; Amino Acid Sequence; Cell Nucleus/metabolism; DNA/chemistry; DNA, Single-Stranded/chemistry; Dose-Response Relationship, Drug; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Exonucleases/metabolism; Gene Deletion; Hydrolysis; Ions; Kinetics; Magnesium/pharmacology; Microscopy, Electron; Molecular Sequence Data; Mutation; Oligonucleotides/chemistry; Protein Binding; Protein Structure, Tertiary; Rec A Recombinases/chemistry; Rec A Recombinases/metabolism; Recombination, Genetic; Surface Plasmon Resonance; Time Factors |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.