PMID:15122345
| Citation |
Nickels, BE, Mukhopadhyay, J, Garrity, SJ, Ebright, RH and Hochschild, A (2004) The sigma 70 subunit of RNA polymerase mediates a promoter-proximal pause at the lac promoter. Nat. Struct. Mol. Biol. 11:544-50 |
|---|---|
| Abstract |
The sigma(70) subunit of RNA polymerase plays an essential role in transcription initiation. In addition, sigma(70) has a critical regulatory role during transcription elongation at the bacteriophage lambda late promoter, lambda P(R'). At this promoter, sigma(70) mediates a pause in early elongation through contact with a DNA sequence element in the initially transcribed region that resembles a promoter -10 element. Here we provide evidence that sigma(70) also mediates a pause in early elongation at the lac promoter (plac). Like that at lambda P(R'), the pause at plac is facilitated by a sequence element in the initially transcribed region that resembles a promoter -10 element. Using biophysical analysis, we demonstrate that the pause-inducing sequence element at plac stabilizes the interaction between sigma(70) and the remainder of the transcription elongation complex. Bioinformatic analysis suggests that promoter-proximal sigma(70)-dependent pauses may play a role in the regulation of many bacterial promoters. |
| Links |
PubMed Online version:10.1038/nsmb757 |
| Keywords |
Base Sequence; DNA-Directed RNA Polymerases/physiology; Escherichia coli Proteins/physiology; Lac Operon; Promoter Regions, Genetic; Protein Binding; Protein Subunits/physiology; Sigma Factor/physiology; Transcription, Genetic |
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